Rnalater Ice Ambion

OCT Embedding TheThe OCT Protocol Is Used For Freezing Tissue ...
Styrofoam container containing dry ice and aluminum plate for stability . RNAlater (Ambion-Applied Biosystems) Tissue stored in RNAlater can be stored at 2o -8o or -80o C for the 2 week hold period if necessary (Mod Pathol 2001; 14(2):116-128. ... Return Document

PROCEDURE Placenta Collection DATE ADOPTED REVIEWED BY ...
Freezing into dry ice-chilled ethanol. Hold momentarily as vial freezes before proceeding to slowly submerge the entire specimen. 2) Dry Ice Method: Fill box with pelleted dry ice or broken up block dry ice. NOTE: Collect RNAlater and frozen specimens only if it has been < 30 minutes after delivery of the placenta. ... Fetch Content

Martins Http://www.veterinaryresearch.org/content/44/1/120 ...
After treatment with RNAlater-ICE (Ambion, Inc, Austin, TX, USA), MLN samples were soaked in RLT buffer (Qiagen, Valencia, CA, USA) and disrupted in a rotor-stator homogenizer. RNA was isolated using the AllPrep DNA/RNA/Protein Mini Kit (Qiagen), digested ... Get Content Here

RNA Stability In Human Liver: Comparison Of Different ...
Sampleswerekept ina containerleft on ice. After the 5 time-points, individual liver samples were flash-frozen in liquid nitrogen for storage at -80 C or put in RNAlater (Ambion, Darmstadt, Germany) and processed for eventual storage at-80 C, according to the manufacturer’s instructions. These frozen samples were then used for subsequent ... Document Retrieval

PARIS™ Kit Protocol (PN 1921M Rev E)
Nitrogen or stored in Ambion® RNAlater® or RNAlater-ICE Solution. Storage in RNAlater Solution will denature proteins, therefore total protein obtained from samples stored in RNAlater or RNAlater-ICE Solution will be competent for applications such as Western blotting or 2D gel electrophoresis, but will not be suitable for applications that ... Fetch Full Source

Latent Gammaherpesvirus 68 Infection Induces Distinct ...
Latent Gammaherpesvirus 68 Infection Induces Distinct Transcriptional Changes in Different Organs from tissues incubated in RNAlater-ICE (Ambion). Equal amounts of RNA ... Retrieve Here

Page 1 Of 40 Diabetes - Diabetes.diabetesjournals.org
Tissue samples suspended in RNALater ICE (Ambion) and stored at -20°C. mRNA was isolated from RNALater ICE stabilized tissues or directly from in vitro cultures of monolayers or cell laden hydrogels with Trizol reagent (Ambion). ... Fetch Doc

CASE PROCESSING 1 PURPOSE 3 RESPONSIBILITIES
RNAlater® (Ambion™), store at 25°C Pipettes and sterile filter tips (200 µl, 1000 µl) Dry ice and ice bucket 2-Methylbutane with dry ice in ice bucket and long forceps 70% ethanol 10-50% Clorox bleach (6% Sodium hypochlorite) Tissue waste container with formalin Sharps containers 5 SAFETY ... Access Doc

Distinct Tissue Cytokine And Chemokine MRNA Expression In ...
Frozen biopsies were immersed in RNAlater Ice (Ambion, Inc., Huntington, UK) cooled to 70 8C and stored at 20 8C for 24 h prior to extraction to improve RNA stability. All biopsy sections were reviewed by a board-certified veterinary histopathologist (M.J.D.), without knowledge of their origin. The samples from control ... Document Retrieval

Expression Of The Wnt Antagonist Dickkopf-3 Is Associated ...
Placed in RNAlater-ICE (Ambion, Austin, TX) at −20 °C. This research did not qualify as human subjects research, due to the lack of personal health information, and thus was considered exempt from IRB approval at UCIMC, as evaluated by the UCIMC IRB board. ... Fetch This Document

Changes In Gene Expression In The Locus Coeruleus-Amygdala ...
Preserved in RNAlater-ICE (Ambion, Austin, TX). Quantitative Real-Time RT-qPCR for mRNA Total RNA was isolated using RNA STAT-60 (Tel-Test, Inc, Friendswood, TX) and RNA concentration was eval-uated by spectrophotometry (NanoDrop 2000, Thermo Fisher Scientific, Pittsburgh, PA). The ratio of absorbance at 260–280 nm was about 2.0. ... Fetch Here

Gene Expression Changes Reflect Clinical Response In A ...
RNAlater-ICE (Ambion, Life Technologies, Grand Island, NY, USA) and RNA prepared for analysis of genome-wide gene expression. Masking Both patients and investigators were blinded to the treat-ment allocation. The same efficacy assessor (LC) per-formed all skin scores and physician global assessments at baseline and week 24, and remained blinded ... Retrieve Full Source

User-Developed Protocol: Preparation Of RNAlater™ Preserved ...
Necessary to cut the tissue on ice or dry ice or in a refrigerated room. The remaining tissue can be stored in RNAlater RNA Stabilization Reagent for subsequent RNA isolation. The reagent preserves RNA for up to 1 day at 37°C, 7 days at 18 to 25°C, or 4 weeks at 2 to 8°C, allowing transportation, storage, and shipping of samples without ice or ... Access Content

CHAPTER 9 Procedures For Quality Control Of RNA Samples For ...
RNAlater-ICEs (Ambion Inc., Applied Biosystems, USA) has been developed to aid tissue processing of previously frozen material. These samples are then processed using conventional column or phenol based systems such as TRIsReagent (Sigma Aldrich, USA). ... Retrieve Doc

TECHNICAL BULLETIN - Sigma-Aldrich
25 C, incubate samples in RNAlater on ice for a few hours if possible before storing at ambient temperature. 5. Storage at 37 C RNA isolated from samples stored at 37 C is intact after a 24 hour incubation, but is partially degraded after a 3 day incubation. C. RNA Isolation from Material in RNAlater ... Access Full Source

Www.life.illinois.edu
Night with RNAlater-ice (Ambion) and then dissected over ice. Only the calyces of the mushroom bodies were retained because of the difficulty of separating the peduncles and lobes of the mushroom bodies from the central body. The calyces contain the somata of all intrinsic mushroom body neurons (Kenyon cells) and should contain the majority of ... Access Full Source